Alafaya Clinic

Our current partnership with Orange County Animal Services allows us to perform low-cost spaying and neutering at our new Alafaya Clinic. Vaccines as well as heartworm testing and prevention will be available for patients at an additional cost.

The clinic was implemented to address a problem plaguing Orange County for decades – pet overpopulation. Each year, Animal Services, the county’s only open admission shelter receives approximately 18,000 homeless pets. While the shelter makes every effort to secure a live release for these pets, due to the sheer volume that is not always possible. Accessibility of spay and neuter services will be key in helping to curb this problem and is a step forward for animal welfare in Orange County.

The clinic will also  focus on the community cat initiative of trap, neuter return by spaying and neutering cats in the community and returning them to their homes.

How May We Help You?

Hover over the boxes for more information.
*New spay/neuter appointments will be made available every third Tuesday of the month by 6:00PM.
*Please note all surgeries require a $60 deposit at the time of scheduling. All deposits are forfeited if surgeries are not cancelled within 48 hours of their scheduled date and time.
*All surgeries must be paid for in full when you drop off your pet (we can take payment onsite morning of). Failure to pay for the surgery fees will result in rescheduling or cancellation.

Spay & Neuter Surgery

A $60 non-refundable deposit is required. Surgeries are by appointment only on Tuesdays and Thursdays. New appointments will be made available every third Tuesday of the month by 6:00PM.

Spay & Neuter Surgery

Animals must be dropped off between 7:15am – 7:45am and picked up between 4:30pm – 5:00pm

Community Cat Day

Friday we are closed to the public to concentrate on our community cat initiative.

Community Cat Day

The clinic will also spay & neuter cats that are part of our TNR program. Pet Alliance of Greater Orlando's goal is to reduce the amount of kittens and cats entering shelters across the county.

Vaccination Clinic

Vaccines are by appointment only on Wednesdays.


Alafaya Clinic
12050 E. Colonial Drive
Orlando, FL 32826


Alafaya Clinic FAQ

Our hours of operation are closely tied to the services we provide. Please see below:

Monday/Saturday – Closed

Spay & Neuter Surgery Days


Tuesday & Thursday – 7:15 a.m. (Animal Drop Off) – 4:00 p.m. (Animal Pick Up) 

New appointments will be made available every third Tuesday of the month by 6:00PM.

Community Cat Day

Friday – Closed to the public

Vaccine Clinic


Wednesday – 7am – 4pm

Yes. An appointment is required at this time for Spay and Neuter Surgeries as well as our Vaccine Clinic.

To schedule an appointment, click the schedule appointment button.

Yes, anyone that is 18 years or older is welcome to make an appointment for their cat(s) or dog(s) to be seen at the clinic.  We offer basic services.  Pricing can be found here.

A handwritten prescription from your Pet Alliance Clinic is needed for online pet pharmacies and other prescription services. Prescriptions MUST be picked up in person (they cannot be faxed, emailed, or mailed). Please call our customer care team at (407) 351-7722 to confirm and pick up your pet’s prescription. You can also use our online pharmacy to order your pet’s medications.

The Pet Alliance Partners with  CareCredit and Scratch Pay.Applications are encouraged to be submitted before arriving at our clinic.

We do not provide boarding services at any of our clinic or shelters.

As animals age there is the potential for development of conditions that can make routinely used anesthesia drugs difficult for their system to handle.  Blood work can help identify problems that should be considered before anesthesia is administered and helps lower the risk of potential complications.

If your animal is over 6 years of age, please call us at (407) 351.7722, as we will book your appointment at our Sanford clinic (map).

It is not required, but strongly encouraged. Spay/Neuter of pets helps to control over population of animals in our area. The result is fewer homeless pets in our community.  If your pet is not altered prior to their first appointment, we can schedule a spay or neuter appointment for you if you would like.

At 8 weeks of age, your puppy should receive Bordatella, DA2PP, and Canine Flu vaccines. It’s also recommended that puppies also receive Strongid, a dewormer.

At 12 weeks of age, your  puppy is old enough to receive their first Rabies and Leptospirosis vaccines. This is also the age where your puppy is able to be spayed/neutered— a surgery that requires proof that a rabies vaccine was given to your animal. 

Boosters for certain shots are required, so know that multiple visits are needed to fully protect your puppy against common diseases. You can make your appointment at any of our clinic by calling (407) 351 – 7722.

Between 6-8 weeks of age your kitten should receive the first FVRCP vaccine, a FVRCP booster at 10-12 weeks, and the third booster at 14-16 weeks. The rabies vaccine should be given at 12-16 weeks of age.

At this time, we do not offer euthanasia services at either clinic.


Due to technical difficulties, Pet Alliance’s call center is closed today, June 14.